How Whey Protein Enhances Athletic Performance and Recovery

How Whey Protein Enhances Athletic Performance and Recovery

 

How Whey Protein Enhances Athletic Performance and Recovery

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In the world of sports nutrition, whey protein stands out as a powerhouse supplement. Athletes and fitness enthusiasts alike have embraced this protein source for its potential to enhance athletic performance and aid in recovery. Whey protein has gained popularity as a by-product of cheese production due to its high-quality amino acid profile and rapid absorption rate, making it a go-to choice for those looking to support muscle growth and improve their overall fitness.

This article delves into how whey protein influences athletic performance and recovery. It explores the science behind whey protein's role in muscle development, post-workout recuperation, and strength gains. Additionally, the piece examines how whey protein can boost endurance exercise performance and provides insights on optimal timing and dosage for athletes. By understanding the benefits and proper use of whey protein, readers can decide to incorporate this supplement into their training regimens and healthy diets.

What is Whey Protein?

Whey protein is a high-quality protein derived from cow's milk, specifically from the liquid portion that separates during cheese production <sup>[1]</sup>. This protein-rich substance has gained significant popularity among athletes, bodybuilders, and fitness enthusiasts due to its numerous health benefits and higher protein content compared to natural foods <sup>[2]</sup>.

The process of creating whey protein involves extracting it from milk, separating it from carbohydrates and fats, and then drying it into a powder form <sup>[2]</sup>. This powder is often flavored to enhance its taste, as whey protein on its own doesn't have a particularly appealing flavor <sup>[1]</sup>. Popular flavors include chocolate, vanilla, and strawberry <sup>[1]</sup>.

One of the key reasons for whey protein's popularity is its impressive amino acid profile. It contains all nine essential amino acids, making it a complete protein source <sup>[3]</sup>. Whey protein is particularly rich in leucine and cysteine, two amino acids that play crucial roles in muscle growth and antioxidant production, respectively <sup>[1]</sup>.

Types of Whey Protein

There are three main types of whey protein, each differing in their processing methods and nutritional profiles:

  1. Whey Protein Concentrate (WPC): This is the most common and cost-effective form of whey protein. It contains about 70-80% protein, with the remaining content being lactose (milk sugar) and fat <sup>[1]</sup>. WPC is known for its superior flavor and is often recommended for beginners or those looking to build muscle or gain weight <sup>[2]</sup>.
  2. Whey Protein Isolate: This type has undergone further processing to remove most of the fat and lactose. It contains 90% protein or higher, making it an excellent choice for those who are lactose intolerant or looking to minimize their carbohydrate and fat intake <sup>[1]</sup>. It's ideal for individuals focusing on developing lean, well-defined muscles <sup>[2]</sup>.
  3. Whey Protein Hydrolysate: This is the most processed form of whey protein. It has undergone partial hydrolysis, a process that breaks down the protein into smaller peptides, making it easier and faster to digest <sup>[1]</sup>. While it's the most expensive option, it's highly beneficial for nutrition support and immune system enhancement <sup>[2]</sup>.

Nutritional Profile

The nutritional content of whey protein can vary depending on the brand and type. However, a typical scoop (about 19.5 grams) of whey protein powder provides the following nutritional values <sup>[3]</sup>:

  • Calories: 75
  • Protein: 13g
  • Fat: 1g
  • Carbohydrates: 3.5g
  • Sugars: 1g
  • Sodium: 75mg

It's worth noting that whey protein is not typically a significant source of vitamins and minerals unless fortified. However, it does contain a notable amount of calcium, with one scoop providing about 50 milligrams, which is approximately 5% of the Recommended Daily Allowance <sup>[3]</sup>.

Whey protein is generally well-tolerated by most people. However, individuals with lactose intolerance should be cautious, as some forms (particularly concentrates) contain lactose <sup>[1]</sup>. In rare cases, some people may be allergic to whey protein <sup>[1]</sup>.

The recommended dosage of whey protein is typically 1-2 scoops (25-50 grams) per day, often consumed after workouts <sup>[1]</sup>. However, it's always best to consult with a healthcare professional or a registered dietitian to determine the optimal dosage based on individual needs and goals.

How Whey Protein Supports Muscle Growth

Whey protein has gained significant popularity among athletes and fitness enthusiasts due to its remarkable ability to support muscle growth. This high-quality protein source contains a complete profile of essential amino acids (EAAs), which are crucial for stimulating muscle protein synthesis (MPS) and promoting overall muscle development.

Amino Acid Content

Whey protein stands out for its impressive amino acid profile, particularly its high concentration of branched-chain amino acids (BCAAs). These BCAAs, namely leucine, isoleucine, and valine, play a vital role in muscle growth and recovery <sup>[4]</sup>. Among these, leucine has been identified as the most critical amino acid for triggering muscle protein synthesis.

The abundance of EAAs in whey protein is particularly significant because muscle protein synthesis requires the availability of all EAAs <sup>[4]</sup>. While the body can compensate for a shortage of non-essential amino acids (NEAAs) through increased production, a lack of any EAA can limit muscle protein synthesis <sup>[4]</sup>. This makes whey protein an ideal supplement for supporting muscle growth, as it provides all the necessary amino acid precursors in one convenient source.

Leucine and Muscle Protein Synthesis

Leucine, the star player among BCAAs, has a unique role in stimulating muscle protein synthesis. It acts not only as a building block for new muscle tissue but also as a signaling molecule that triggers the process of protein synthesis <sup>[5]</sup>. Research has shown that leucine can activate the mammalian target of the rapamycin (mTOR) signaling pathway, which is a key regulator of muscle protein synthesis <sup>[6]</sup>.

To effectively stimulate muscle protein synthesis, it's crucial to consume a threshold amount of leucine, typically around 3-5 grams, depending on body weight <sup>[5]</sup>. Whey protein is particularly rich in leucine, with approximately 10% of its protein content being leucine <sup>[5]</sup>. This means that a standard 20-25g scoop of whey protein provides about 2-2.5g of leucine, which is close to the threshold required to trigger the anabolic response.

Studies have demonstrated that leucine supplementation can enhance total lean body mass and muscle strength, particularly in older individuals <sup>[6]</sup>. This suggests that the high leucine content in whey protein may be especially beneficial for maintaining and building muscle mass as we age.

It's worth noting that while leucine is crucial, the presence of all EAAs is necessary for sustained muscle protein synthesis <sup>[4]</sup>. Whey protein provides this complete amino acid profile, making it an excellent choice for supporting muscle growth.

The effectiveness of whey protein in promoting muscle growth is not just due to its amino acid content, but also its rapid absorption rate. This quick digestion and absorption lead to a fast increase in plasma amino acid levels, which can enhance the muscle protein synthesis response, especially when consumed after resistance exercise <sup>[7]</sup>.

In conclusion, whey protein supports muscle growth through its rich amino acid profile, particularly its high leucine content. By providing all the necessary EAAs and triggering the mTOR pathway through leucine, whey protein creates an optimal environment for muscle protein synthesis. This makes it a valuable supplement for individuals looking to enhance their muscle growth and recovery, whether they're athletes, bodybuilders, or simply fitness enthusiasts aiming to improve their body composition.


Whey Protein's Role in Post-Workout Recovery

Whey protein has gained significant attention in the realm of sports nutrition for its potential to enhance post-workout recovery. This high-quality protein source has been shown to have a positive impact on various aspects of recovery, including muscle repair and glycogen replenishment.

Reducing Muscle Damage

Exercise-induced muscle damage (EIMD) is a common occurrence following intense workouts, particularly resistance training. EIMD can lead to reduced muscle function, soreness, and swelling, which may persist for up to seven days post-exercise <sup>[8]</sup>. Whey protein supplementation has emerged as a promising strategy to mitigate these effects and accelerate recovery.

Research has demonstrated that consuming whey protein after exercise can help maintain maximal strength and lower creatine kinase concentration, a marker of muscle damage <sup>[8]</sup>. This effect is attributed to whey protein's ability to stimulate muscle protein synthesis (MPS), which is crucial for repairing damaged muscle proteins <sup>[9]</sup>.

The timing of whey protein intake plays a crucial role in its effectiveness. Studies have shown that consuming protein within the first two hours after exercise can be particularly beneficial for stimulating muscle repair and growth <sup>[9]</sup>. The International Society of Sports Nutrition (ISSN) recommends consuming 20 to 40 grams of protein every 3 to 4 hours to support muscle recovery and improve body composition <sup>[9]</sup>.

However, it's important to note that the benefits of whey protein supplementation on muscle damage markers may vary depending on the specific circumstances. Some studies have found that a single dose of whey protein supplement, regardless of timing, may not significantly affect muscle damage markers after eccentric exercise <sup>[10]</sup>. This suggests that consistent protein intake throughout the day, rather than relying solely on post-workout supplementation, may be more effective for long-term recovery and adaptation.

Replenishing Glycogen Stores

In addition to its role in muscle repair, whey protein can also contribute to the replenishment of glycogen stores, which are crucial for energy during exercise. While carbohydrates are the primary nutrient for glycogen synthesis, the addition of protein to post-workout nutrition can enhance this process under certain conditions.

Glycogen synthesis following exercise occurs in two distinct phases: a rapid phase lasting 30-60 minutes that doesn't require insulin, and a slow phase that can last several hours <sup>[11]</sup>. The timing of nutrient intake is critical, as delaying carbohydrate consumption by two hours after prolonged exercise can decrease muscle glycogen synthesis by 45% <sup>[11]</sup>.

While carbohydrates play the primary role in glycogen replenishment, the addition of whey protein to post-workout nutrition can provide additional benefits. Research suggests that consuming a combination of protein and carbohydrates after exercise is the most effective way to maximize recovery and prevent injury <sup>[9]</sup>. The ISSN recommends consuming 0.4 grams of carbohydrates per pound of body weight (0.8 g/kg) along with 0.1 to 0.2 grams of protein per pound of body weight (0.2 to 0.4 g/kg) during each hour within the first four hours post-exercise to optimize glycogen synthesis <sup>[9]</sup>.

It's worth noting that the type and form of carbohydrates consumed alongside whey protein can influence the rate of glycogen synthesis. High glycemic index (GI) carbohydrates are more effective in replenishing glycogen stores in the early hours following exercise <sup>[11]</sup>.

In conclusion, whey protein plays a multifaceted role in post-workout recovery. Its ability to support muscle repair and contribute to glycogen replenishment makes it a valuable component of post-exercise nutrition strategies. By incorporating whey protein into their recovery regimen, athletes and fitness enthusiasts can potentially enhance their body's ability to recover from intense workouts and prepare for subsequent training sessions.

Whey Protein and Strength Gains

Whey protein supplementation has gained significant attention in the fitness world for its potential to enhance strength gains and improve overall athletic performance. Research has shown that whey protein when combined with resistance exercise training (RET), can have a positive impact on muscle mass and strength development.

Impact on Muscle Mass

Studies have demonstrated that whey protein supplementation, particularly when consumed before and after resistance exercise, can lead to significant improvements in muscle hypertrophy <sup>[12]</sup>. The high leucine content in whey protein, which is 50-75% more than other protein sources, plays a crucial role in stimulating muscle protein synthesis and upregulating the mammalian target of rapamycin (mTOR) signaling pathway <sup>[13]</sup>. This process is essential for muscle adaptation and growth.

Whey protein hydrolysate (WPH) has emerged as one of the most common types of protein supplements used in conjunction with resistance training <sup>[12]</sup>. Its effectiveness is attributed to its ability to induce protein synthesis by increasing components of the anabolic hormone response, such as growth hormone (GH), insulin-like growth factor (IGF-1), and testosterone <sup>[12]</sup>.

Research has shown that consuming approximately 20 grams of protein after resistance exercise is sufficient to stimulate muscle protein synthesis <sup>[12]</sup>. However, the timing of protein intake is equally important. Studies have found that consuming whey protein immediately before and after resistance exercise sessions has a beneficial effect on muscle hypertrophy <sup>[12]</sup>.

A study conducted on mice demonstrated that whey protein supplementation, when combined with exercise training, resulted in greater improvements in body composition and biochemical assessments compared to exercise alone <sup>[13]</sup>. The researchers observed that whey protein supplementation led to increased grip strength and levels of albumin and total protein, indicating enhanced muscle strength and overall protein status <sup>[13]</sup>.

Effects on Power Output

Whey protein supplementation has also shown promising results in improving power output and overall muscular strength. A study examining the effects of whey protein on resistance exercise-induced increases in muscle mass and strength found significant enhancements in overall muscular strength and endurance, independent of dietary influence <sup>[14]</sup>.

Researchers assessed peak torque at 60°/s during joint flexion-extension and total work at 180°/s during joint flexion-extension to measure improvements in strength and power output <sup>[14]</sup>. These assessments provided valuable insights into the impact of whey protein on muscular performance.

Another study found a moderate beneficial effect on acute anaerobic power and strength in the group that consumed a protein supplement, suggesting potential improvements in the rate of recovery compared to those who consumed a carbohydrate drink <sup>[15]</sup>. This finding highlights the importance of protein quality in both acute and chronic adaptations to exercise.

It's worth noting that the benefits of whey protein supplementation extend beyond anaerobic activities. Recent research has explored its role in endurance activities as well. A systematic review and meta-analysis comparing 11 studies investigated the effects of consuming protein and carbohydrate versus carbohydrate alone during cycling on subsequent performance <sup>[15]</sup>. The results showed an average improvement of 9% in performance (defined as time to exhaustion and time trial performance) when protein and carbohydrate were consumed together <sup>[15]</sup>.

While the improvements in time trial performance were not statistically significant, studies utilizing time-to-exhaustion protocols did show statistically significant improvements <sup>[15]</sup>. Interestingly, in all studies showing statistically significant improvement, whey protein was the source of protein utilized <sup>[15]</sup>.

These findings underscore the potential of whey protein supplementation in enhancing both strength and endurance performance. Even seemingly small improvements can have significant effects on performance and outcomes at the elite level. For instance, a 1% improvement in performance could mean the difference between winning a gold medal instead of a silver medal in an Olympic event <sup>[15]</sup>.


Enhancing Endurance Performance with Whey

While whey protein is often associated with strength training and muscle growth, its benefits extend to endurance performance as well. Recent research has shed light on how whey protein supplementation can enhance endurance athletes' performance and recovery.

Delaying Fatigue

One of the key ways whey protein supports endurance performance is by delaying fatigue. Studies have shown that consuming whey protein during endurance exercise can have a positive impact on performance and recovery. A systematic review and meta-analysis of 11 studies found that consumption of protein and carbohydrates during cycling resulted in an average improvement of 9% in performance compared to carbohydrate alone <sup>[16]</sup>.

The benefits of whey protein for endurance athletes go beyond mere caloric intake. When researchers examined isocaloric conditions, they still observed a 3.4% improvement in performance <sup>[16]</sup>. This suggests that the unique properties of whey protein, rather than just additional calories, contribute to enhanced endurance performance.

Branched-chain amino acids (BCAAs), which are abundant in whey protein, play a crucial role in delaying central fatigue during endurance exercise. Research has shown that BCAA consumption during endurance exercise may improve time trial performance and peak power output <sup>[17]</sup>. Additionally, BCAAs have been found to attenuate serotonin levels, which can delay the onset of central fatigue <sup>[1]</sup>.

Supporting Energy Production

Whey protein also supports endurance performance by aiding in energy production and recovery. During prolonged exercise lasting beyond two hours, the body may begin to break down muscle tissue for energy if adequate protein is not provided. This process, known as muscle cannibalization, can lead to increased fatigue and impaired recovery <sup>[18]</sup>.

To prevent this, endurance athletes are advised to consume both complex carbohydrates and protein during extended exercise sessions. Whey protein, with its high biological value and rapid absorption rate, is particularly well-suited for this purpose <sup>[18]</sup>.

Moreover, whey protein has been shown to have beneficial effects on biochemical markers of metabolism and muscle damage in endurance athletes. A study on elite marathon runners found that those who supplemented with whey protein (33.5 g/day) during their training had lower levels of creatine kinase (CK) and lactate dehydrogenase (LDH) post-marathon compared to those who consumed maltodextrin <sup>[19]</sup>. These lower levels of muscle damage markers suggest improved recovery and potentially better subsequent performance.

The same study also found that whey protein supplementation led to decreased triglycerides and total cholesterol post-marathon <sup>[19]</sup>. This improved lipid profile may indicate a more efficient conversion of cholesterol to steroid hormones, potentially enhancing physiological recovery and adaptations from strenuous exercise.

Interestingly, the benefits of whey protein supplementation for endurance athletes extend beyond the immediate post-exercise period. One week after a marathon, runners who had supplemented with whey protein showed better performance in a 12-minute run/walk test compared to those who had consumed maltodextrin <sup>[19]</sup>. This suggests that whey protein may have long-lasting effects on recovery and subsequent performance for endurance athletes.

In conclusion, whey protein has emerged as a valuable supplement for endurance athletes, offering benefits in delaying fatigue, supporting energy production, and enhancing recovery. Its unique amino acid profile, particularly its high BCAA content, contributes to improved endurance performance and reduced muscle damage. As research in this area continues to evolve, endurance athletes may increasingly turn to whey protein as a key component of their nutritional strategy.


Optimal Timing of Whey Protein Intake

The timing of whey protein intake has been a subject of considerable debate in the fitness and sports nutrition communities. For years, the concept of an 'anabolic window' has dominated discussions about post-workout nutrition. However, recent research has challenged this notion, suggesting that the optimal timing for whey protein consumption may be more flexible than previously thought.

Pre-Workout Benefits

Consuming whey protein before a workout has gained attention for its potential benefits. Research suggests that it doesn't matter whether one drinks a protein shake before or after a workout in terms of optimizing muscle repair and growth <sup>[20]</sup>. This finding challenges the long-held belief that post-workout protein intake is superior.

Pre-workout protein consumption can provide several advantages:

  1. Increased amino acid availability during exercise
  2. Potential reduction in muscle protein breakdown
  3. Enhanced recovery process

A study comparing the effects of consuming protein either before or after a workout on muscle strength and size found no significant differences between the groups <sup>[20]</sup>. This indicates that as long as protein is consumed around the workout, the timing may not be critical.

Post-Workout Window

The concept of the 'anabolic window' suggests that there is a short period after exercise during which nutrient intake is crucial for maximizing muscle growth and recovery. Traditionally, this window was thought to last only 30 minutes post-exercise <sup>[21]</sup>.

However, recent research has challenged this narrow timeframe:

  1. The anabolic response to exercise may last much longer than initially believed.
  2. The urgency of immediate post-workout protein intake may be overstated, especially if a pre-workout meal is consumed.
  3. Total daily protein intake appears to be more important than precise timing for muscle growth and strength gains <sup>[20]</sup>.

A review published in the Journal of the International Society of Sports Medicine found that the window for protein ingestion is actually much wider - think hours, not minutes <sup>[16]</sup>. The main focus should be on total daily protein intake rather than precise timing.

For those who train in a fasted state, immediate post-workout nutrition becomes more critical. A 2003 study showed that fasted exercise significantly increases post-training muscle breakdown <sup>[21]</sup>. In such cases, consuming protein soon after the workout may be beneficial.

Practical Recommendations:

  1. Consume high-quality protein dosed at 0.4–0.5 grams per kilogram of lean body mass, both before and after exercise <sup>[16]</sup>.
  2. Focus on meeting daily protein requirements rather than strict timing.
  3. If training in a fasted state, prioritize post-workout protein intake.
  4. Choose whey protein for its rapid absorption rate, making it useful both before and after workouts <sup>[20]</sup>.

In conclusion, while the timing of whey protein intake can influence muscle protein synthesis and recovery, the effects are not as time-sensitive as once believed. The most important factor appears to be consistent, adequate protein intake throughout the day. Athletes and fitness enthusiasts have the flexibility to consume whey protein at times that best suit their individual preferences and schedules, as long as they meet their overall nutritional needs.

Recommended Dosage for Athletes

Daily Protein Requirements

The optimal protein intake for athletes has been a subject of extensive research and debate. While the current Recommended Dietary Allowance (RDA) for protein is 0.8 g/kg body weight for sedentary adults, athletes require significantly higher amounts to support their training and recovery needs <sup>[22]</sup>.

For athletes engaged in regular exercise, the International Society of Sports Nutrition (ISSN) recommends a daily protein intake ranging from 1.4 to 2.0 g/kg of body weight <sup>[23]</sup>. This recommendation aligns with the Acceptable Macronutrient Distribution Range published by the Institute of Medicine for Protein <sup>[23]</sup>.

The specific protein requirements can vary based on the type of athletic activity:

  1. Endurance athletes: 1.0-1.8 g/kg body weight <sup>[22]</sup>
  2. Strength/Power athletes: 1.4-2.0 g/kg body weight <sup>[22]</sup>
  3. Athletes in a weight-loss period: 1.6-2.4 g/kg body weight <sup>[22]</sup>

It's important to note that these recommendations can be influenced by factors such as training status, exercise intensity, exercise duration, and overall dietary intake <sup>[22]</sup>.

For recreational athletes primarily engaged in low- to moderate-intensity endurance exercise, studies have shown that their protein needs may not differ significantly from those of sedentary individuals <sup>[22]</sup>. However, as training intensity and frequency increase, so do protein requirements.

Modestly trained athletes may require approximately 37.5% more protein than the RDA, which translates to about 1.1 g/kg body weight <sup>[22]</sup>. Elite endurance athletes have shown optimal results with protein intakes ranging from 1.5 to 1.8 g/kg body weight <sup>[22]</sup>.

For strength and power athletes, regular resistance exercise creates a need for greater protein availability to recover from the stress and trauma of training <sup>[22]</sup>. A meta-analysis of 22 studies demonstrated that protein supplementation has a positive impact on improvements in fat-free mass and leg strength in both young and old populations <sup>[22]</sup>.

Interestingly, as athletes become more trained, their bodies may process protein more efficiently. Some studies suggest that more experienced athletes might have slightly lower protein requirements compared to novices <sup>[22]</sup>.

Per-Serving Guidelines

While daily protein intake is crucial, the timing and distribution of protein consumption throughout the day also play a significant role in maximizing muscle protein synthesis (MPS) and recovery.

General recommendations for optimal protein intake per serving are:

  1. 0.25-0.3 g of high-quality protein per kg of body weight <sup>[17]</sup>
  2. An absolute dose of 20-40 g <sup>[23]</sup>

These protein doses should ideally be evenly distributed every 3-4 hours across the day <sup>[23]</sup>. This strategy helps maintain a consistent anabolic environment, supporting muscle growth and recovery.

For post-exercise nutrition, consuming 0.25-0.3 g of high-quality protein per kg of body weight (typically 15-25 g for most individuals) within 0-2 hours after exercise has been shown to increase muscle protein synthesis <sup>[17]</sup>. This same amount is recommended every 3-5 hours over multiple meals throughout the day to maximize muscular adaptation <sup>[17]</sup>.

It's worth noting that acute protein doses should aim to contain 700-3000 mg of leucine and/or a higher relative leucine content, along with a balanced array of essential amino acids (EAAs) <sup>[23]</sup>. Whey protein, being rich in leucine, is particularly effective for this purpose.


Conclusion

Whey protein has proven to be a game-changer in the world of sports nutrition. Its ability to support muscle growth, enhance recovery, and boost both strength and endurance performance makes it a valuable tool for athletes across various disciplines. The optimal use of whey protein involves considering factors such as timing, dosage, and individual nutritional needs to maximize its benefits.

To wrap up, the key to harnessing the full potential of whey protein lies in understanding its role within a comprehensive nutrition and training plan. By incorporating whey protein strategically, athletes can enhance their performance, speed up recovery, and work towards achieving their fitness goals more effectively. As research in this field continues to evolve, whey protein remains a cornerstone supplement for those looking to optimize their athletic performance and overall health.

FAQs

How does whey protein aid in recovery after exercise?
Whey protein is highly effective in enhancing recovery post-exercise by boosting protein synthesis and minimizing muscle damage, as evidenced by various studies (Buckley et al., 2010; Cooke et al., 2010).

In what ways does protein assist athletes with recovery?
Protein, particularly high-quality types that include all essential amino acids and are abundant in branched-chain amino acids (BCAAs), significantly assists in muscle recovery and growth. Leucine, a BCAA, is especially crucial for muscle development and recovery following both resistance and endurance training.

Can whey protein supplements improve recovery from exercise?
Whey protein, rich in glutamine and BCAAs like isoleucine and valine, with leucine being the most significant for muscle protein synthesis, is a favored supplement. Studies confirm that whey protein enhances recovery following resistance training.

What performance benefits does whey protein offer?
Whey protein is an exceptional resource for muscle development, providing essential amino acids needed for muscle growth, tissue repair, and new muscle formation. It also helps in reducing and preventing the degeneration of body tissues.

References

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[14] - https://www.mdpi.com/2072-6643/15/4/1003
[15] - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6142015/
[16] - https://www.menshealth.com/uk/nutrition/a44809941/protein-shake-before-or-after-workout/
[17] - https://www.canr.msu.edu/news/protein_intake_for_athletes
[18] - https://www.hammernutrition.com.au/info-centre/protein-why-its-important-for-endurance-athletes/
[19] - https://www.briancolemd.com/blog-post/blog/whey-to-go-the-power-of-protein-for-athletes/
[20] - https://www.healthline.com/nutrition/protein-shake-before-or-after-workout
[21] - https://www.healthline.com/health/fitness-exercise/anabolic-window
[22] - https://www.scienceforsport.com/how-much-protein-do-athletes-really-need/
[23] -
https://jissn.biomedcentral.com/articles/10.1186/s12970-017-0177-8